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Salt Tolerance in Tomatoes
Herbicide Resistance in Crops
Natalie's Gift for Class of 2011
On Cell Respiration
Neurobiology and Behavior
Microbes and Biotechnology
JUNIORS STUDY TEAMS
Sarah's Sure Shots
Maddie's Mad House
On Cell Respiration
3.7.1 This metabolical process is the controlled release of energy from organic compounds to form ATP (Adenosine Triphosophate). Oxygen is consumed as a reactant along with organic fuel. In eukaryotic cells, mitochondria house most of the metabolic equipment for cellular respiration. All living organisms respire in order for their existence, although the substrates which they use may vary. Cellular respiration principles are similar to the combustion of gas in an automobile engine after oxygen is mixed with the fuel (hydrocarbons). Food provides the fuel for respiration, and the exhaust is carbon dioxide and water
Organic Compunds + Oxygen ---> Energy + Water + Carbon Dioxide
( e.g. glucose)------------------------(ATP + Heat)--------------------------------
3.7.2 In cell respiration, glucose in the cytoplasm is broken down by glycolisis into a pyruvate, with a small yield of ATP. The video below explains and demonstrates the beginning stages of glycolisis.
3.7.3 In the absence of oxygen (anaerobic respiration) the pyruvate remains in the cytoplasm and in humans is converted into lactate which is removed from the cell. In yeast, the pyruvate is converted into carbon dioxide and ethanol. In both cases, a small amount of ATP is produced.
3.7.4 When oxygen is present (aerobic respiration), the pyruvate is taken up into the mitochondria and is broken down into carbon dioxide and water. A large amount of ATP is released during this process.
The Image below shows the break down of glucose through anaerobic and aerobic respiration.
8.1.1 Oxidation involves the loss of electrons from an element, whereas reduction involves the gain of electrons and that oxidation frequently involves gaining oxygen or losing hydrogen, whereas reduction frequently involves losing oxygen or gaining hydrogen.
Loss of electrons
Gain of electrons
Gain of oxygen
Loss of oxygen
Loss of hydrogen
Gain of hydrogen
Results in many C-O bonds
Results in many C-H bonds
Results in a compound with lower potential energy
Results in a compound with higher potential energy
The process of glycolysis, including phosphorylation, lysis, oxidation and ATP formation is outlined below
The direct definition of gycolisis is "sugar splitting" . It is the beginning stage of cell respiration
- Glucose is phosphorylated. Phosphorylation is when there are two phosphate groups added to an organic molecule or protein. Hexose biphosphate is the product of the phosphorylation of glucose. Two ATP allows phosphorylation.
Lysis of hexose biphosphate. Hexose biphosphate splits into two molecules of triose phosphate.
Each triose phosphate molecules is oxidised. Two atoms of hydrogen are removed from each molecule. The energy released by the oxidation is used to add another phosphate group to each molecule. This will result in two 3-carbon compounds, each carrying two phosphate groups. NAD+ is the hydrogen carrier that accepts the hydrogen atoms lost from each triose phosphate molecule.
- Two pyruvate molecules are formed by removing two phosphate groups from each molecule. These phosphate groups are given to ADP molecules and form ATP.
Glycolysis occurs in the cytoplasm of cells. Two ATP molecules are used and 4 ATP molecules are produced. Therefore there is a net yield of two ATP molecules. Also, two NAD+ are converted into NADH + H+ during glycolysis.
The diagram below demonstrates glycolisis.
The diagram below shows the structure of a mitochondrion as seen in electron micrographs.
Glycolysis can take place without oxygen. This forms the anaerobic part of cell respiration and therefore is called anaerobic cell respiration. However, the pyruvate produced from glycolysis cannot be oxidised further without the presence of oxygen. The oxidisation of the pyruvate forms part of the aerobic respiration and therefore is called aerobic cell respiration. Aerobic respiration occurs in the mitochondria of cells. The first reaction to take place is the link reaction.
The Link Reaction
Mitochondria in cells take up the pyruvate which is formed from glycolysis in the cytoplasm. Once the pyruvate is in the mitochondrion, enzymes within the matrix of the mitochondrion remove hydrogen and carbon dioxide from the pyruvate. This is called oxidation (removal of hydrogen or addition of oxygen) and decarboxylation (removal of carbon dioxide). Therefore, the process is called oxidative decarboxylation. The hydrogen removed is accepted by NAD+. The link reaction results in the formation of an acetyl group. This acetyl group is then accepted by CoA and forms acetyl CoA.
Below is an image of the link reaction.
The Krebs Cycle
- In the first stage of the Krebs cycle, the acetyl group from acetyl CoA is transferred to a four carbon compound. This forms a six carbon compound.
- This six carbon compound then undergoes decarboxylation (CO2 is removed) and oxidation (hydrogen is removed) to form a five carbon compound. The hydrogen is accepted by NAD+ and forms NADH + H+.
- The five carbon compound undergoes decarboxylation and oxidation (hydrogen is removed) again to form a four carbon compound. The hydrogen is accepted by NAD+ and forms NADH + H+.
The four carbon compound then undergoes substrate-level phosphorylation and during this reaction it produces ATP. Oxidation also occurs twice (2 hydrogens are removed). The one hydrogen is accepted by NAD+ and forms NADH + H+. The other is accepted by FAD and forms FADH2. The four carbon compound is then ready to accept a new acetyl group and the cycle is repeated.
The carbon dioxide that is removed in these reactions is a waste product and is excreted from the body. The oxidations release energy which is then stored by the carriers when they accept the hydrogen. This energy is then later on used by the electron transport chain to produce ATP.
Below is an image of the Krebs Cycle.
Carbon dioxide is removed in two reactions
Hydrogen is removed in 4 reactions
NAD+ accepts the hydrogen in 3 reactions
FAD accepts the hydrogen in 1 reaction
ATP is produced in one of the reactions
The Electron Transport Chain
Inside the inner membrane of the mitochondria there is a chain of electron carriers. This chain is called the electron transport chain. Electrons from the oxidative reactions in the earlier stages of cell respiration pass along the chain. NADH donates two electrons to the first carrier in the chain. These two electrons pass along the chain and release energy from one carrier to the next. At three locations along the chain, enough energy is released to produce ATP via ATP synthase. ATP synthase is an enzyme that is also found in the inner mitochondrial membrane. FADH2 also donates electrons but at a later stage than NADH. Also, enough energy is released at only two locations along the chain by electrons from FADH2. The ATP production relies on energy release by oxidation and it is therefore called oxidative phosphorylation.
Below is an image of the electron transport chain.
The Role of Oxygen
Oxygen is important for cell respiration as at the end of the electron transport chain, the electrons are donated to oxygen. This occurs in the matrix at the surface of the inner membrane. At the same time oxygen binds with hydrogen ions and forms water.
If there is no oxygen then electrons can no longer pass through the electron transport chain and NADH + H+ can no longer be reconverted into NAD+. Eventually NAD+ in the mitochondrion runs out and therefore the link reaction and Krebs cycle no longer take place.
Oxidative phosphorylation in terms of chemiosmosis:
When electrons pass through the electron transport chain they release energy. This energy is then used to pump protons (H+) from the matrix across the inner mitochondrial membrane and into the space between the inner and outer mitochondrial membranes. The space between the inner and outer membranes has a small volume and therefore as the protons move across they create a concentration gradient very quickly. This process is called chemiosmosis. There is now a high concentration of protons in the space between the inner and outer membranes and a low concentration of protons in the matrix.
The image shows the movement of protons from the matrix into the space between the inner and outer membranes. This creates a concentration gradient. The energy used to pump these protons across the inner membrane comes from the energy released by the electrons passing through the electron transport chain
The protons then move down the concentration gradient from the space between the inner and outer membranes back into the matrix. However, they can only move back across via an enzyme embedded in the inner membrane. This enzyme is called ATP synthase. The protons are transported back into the matrix through the channels of ATP synthase and as they do so they release energy. This energy is then used by ATP synthase to convert ADP into ATP. Since the electrons come from previous oxidation reactions of cell respiration and the ATP synthase catalyses the phosphorylation of ADP into ATP, this process is called oxidative phosphorylation. Chemiosmosis is necessary for oxidative phosphorylation to work.
The image shows shows the movement of protons down their concentration gradient. They can only travel through the inner membrane via ATP synthase and as they do so they release energy. This energy is used by ATP synthase to convert ADP into ATP (oxidative phosphorylation).
The relationship between the structure of mitochondrion and its structure:
Watery substance that contains ribosomes and many enzymes. These enzymes are vital for the link reaction and the Krebs Cycle.
The electron transport chain and ATP synthase are found in this membrane. These are vital for oxidative phosphorylation.
Space between Inner and Outer Membrane
Small volume space into which protons are pumped into. Due to its small volume, a high concentration gradient can be reached very quickly. This is vital for chemiosmosis.
This membrane separates the contents of the mitochondrion from the rest of the cell. It creates a good environment for cell respiration.
These tubular projections of the inner membrane increase the surface area for oxidative phosphorylation.
. N.p., 2010. Web. 12 Mar 2011.
Cambell, Neil, and Jane Reece.
. Seventh. San Francisco, CA: Pearson Benjamin Cummings, 2005. 160-80. Print.
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